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You are here: Home / Topics / Genetics / Why even 17th edition Harrison is important as far as entrance exam is concerned??? Genetics from Positive OHC

Why even 17th edition Harrison is important as far as entrance exam is concerned??? Genetics from Positive OHC

August 11, 2012 by TargetPG 1 Comment

Why even 17th edition is important as far as entrance exam is concerned??? Genetics from Positive OHC

Why even 17th edition Harrison is important as far as entrance exam is concerned??? Genetics from Positive OHC
-oOo-

Should you read 17th Edition Harrison for AIIMS Nov 2012, PGI Dec 2012, AIPG 2012
or
Should you read 18th Edition Harrison
or

Both

Or is there a way to answer these question without reading the whole of both editions of Harrison

See the Answer to this question at the end of this discussion

Why even 17th edition Harrison is important as far as entrance exam is concerned??? Genetics from Positive OHC

Why even 17th edition Harrison is important as far as entrance exam is concerned??? Genetics from Positive OHC
Why even 17th edition Harrison is important as far as entrance exam is concerned??? Genetics from Positive OHC

Genetics – A Table from 18TH EDITION Harrison

METHOD PRINCIPLE MUTATION DETECTED
Cytogenetic analysis Unique visual appearance of various chromosomes Numerical or structural abnormalities in chromosomes
Fluorescent in situ hybridization (FISH) Hybridization to chromosomes with fluorescently labeled probes Numerical or structural abnormalities in chromosomes
Southern blot Hybridization with genomic probe or cDNA probe after digestion of high molecular DNA Large deletion, insertion, rearrangement, expansions of triplet repeat, amplification

METHOD

PRINCIPLE

MUTUAL  DETECTED

Polymerase chain reaction (PCR)

Amplification of DNA segment

Expansion of triplet repeats, variable number of tandem repeats (VNTR), gene rearrangements, translocations; prepare DNA for other mutation methods

Reverse transcriptase PCR (RT-PCR)

Reverse transcription, amplification of DNA segment absence or reduction of mRNA transcription

Analyze expressed mRNA (cDNA) sequence; detect loss of expression

Traditional DNA sequencing

Direct sequencing of PCR products

Sequencing of DNA segments cloned into plasmid vectors

Point mutations, small deletions and insertions

METHOD

PRINCIPLE

MUTATION  DETECTED

Next-generation DNA sequencing

Sequencing of large contiguous genomic regions, exome of single or all chromosomes.

Deep sequencing for detection of mutations and SNPs. Sequencing of whole genomes of microorganisms

Microarrays

Hybridization of PCR products to wild-type or mutated oligonucleotides

Point mutations, small deletions and insertions

Genotyping of SNPs

A table from genetics in 17th edition Harrison 

METHOD

PRINCIPLE

MUTATION  DETECTED

Cytogenetic analysis

Unique visual appearance of various chromosomes

Numerical or structural abnormalities in chromosomes

Fluorescent in situ hybridization (FISH)

Hybridization to chromosomes with fluorescently labeled probes

Numerical or structural abnormalities in chromosomes

Southern blot

Hybridization with genomic probe or cDNA probe after digestion of high molecular DNA

Large deletion, insertion, rearrangement, expansions of triplet repeat, amplification

Polymerase chain reaction (PCR)

Amplification of DNA segment

Expansion of triplet repeats, variable number of tandem repeats (VNTR), gene rearrangements, translocations; prepare DNA for other mutation methods

 

METHOD

PRINCIPLE

MUTATION DETECTED

Reverse transcriptase PCR (RT-PCR)

Reverse transcription, amplification of DNA segment absence or reduction of mRNA transcription

Analyze expressed mRNA (cDNA) sequence; detect loss of expression

DNA sequencing

Direct sequencing of PCR products

Point mutations, small deletions and insertions

Sequencing of DNA segments cloned into plasmid vectors

Restriction fragment polymorphism (RFLP)

Detection of altered restriction pattern of genomic DNA (Southern blot) or PCR products

Point mutations, small deletions and insertions

Single-strand conformational polymorphism (SSCP)

PCR of DNA segment: Mutations result in conformational change and altered mobility

Point mutations, small deletions and insertions

 

METHOD

PRINCIPLE

MUTATION DETECTED

Denaturing gradient gel electrophoresis (DGGE)

PCR of DNA segment: Mutations result in conformational change and altered mobility

Point mutations, small deletions and insertions

RNAse cleavage

Cleavage of mismatch between mutated and wild-type sequence

Point mutations, small deletions and insertions

Oligonucleotide specific hybridization (OSH)

Hybridization of PCR products to wild-type or mutated oligonucleotides immobilized on chips or slides

Point mutations, small deletions and insertions

Microarrays

Hybridization of PCR products to wild-type or mutated oligonucleotides

Point mutations, small deletions and insertions

Genotyping of SNPs

 

METHOD

PRINCIPLE

MUTATION DETECTED

Protein truncation test (PTT)

Transcription/translation of cDNA isolated from tissue sample

Mutations leading to premature truncations

Pyrosequencing

Clonal amplification of single DNA fragments on microparticles followed by massive parallel sequencing

Sequencing of whole genomes of microorganisms, resequencing of amplicons

Multiplex ligation-dependent probe amplification (MLPA)

Quantification of PCR-generated amplicons reflecting the number of copies of a specific DNA sequence

Copy number variations

 

All these are methods for assessing genetic mutations except AIIMS NOV-2011

  1. ü A)SSCP
  2. ü B) Denaturing gradient gel electrophoresis (DGGE)
  3. ü C) DNA sequencing
  4. ü D)agarose gel electrophoresis

ANS-D

This clearly depicts the relevance of 17th edition Harrison for entrance exams.So,OHC will be completely based on 18th edition of Harrison,but judiciously it will retain the vital entrance related points which were left out from the 17th edition Harrison in the 18th Edition !!

What is in store for tomorrow???-A new point  from 18th edition of HARRISON regarding LEUCOCYTE ADHESION DEFICIENCY-Kindly log on tomorrow

-oOo-

What are the various questions that are being asked from Genetics in AIIMS, PGI and AIPG and How to answer them correctly ?

  • Reading the complete Harrison is one method.

  • Attending OHC : Crash Course for AIIMS, PGI and AIPG is another method

Filed Under: Genetics Tagged With: Coaching, OHC - Operation Harri Capsule : Crash Course for AIIMS PGI and AIPG Exams, Pozitive Positive

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Comments

  1. himanshushekhar says

    August 13, 2012 at 11:35 pm

    Good n innovating

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